Interesting Papers
Cardiac Development and Therapy
Stem cell, development, markers
1. Palpant, N. J. et al. Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells. Nat Protoc 12, 15–31 (2017).
Overall Cardiac Development
1. Laflamme, M. A. & Murry, C. E. Heart regeneration. Nature 473, 326–335 (2011).
Statistics on Cardiac Disease and Heart Failure
1. Mozaffarian, D., et. al., (2016) Heart Disease and Stroke Statistics-2016 Update: A Report From the American Heart Association. Circulation. 133, e38–360
2. CDC Stats on Heart Disease: https://www.cdc.gov/heartdisease/facts.htm
Drug Testing and Analysis
1. Sharma, A., McKeithan, W. L., Serrano, R., Kitani, T., Burridge, P. W., del Álamo, J. C., Mercola, M., and Wu, J. C. (2018) Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity. Nat Protoc. 13, 3018–3041
2. Nair, A., and Jacob, S. (2016) A simple practice guide for dose conversion between animals and human. J Basic Clin Pharma. 7, 27
Anesthesia and Pain Medicine
Drug Mechanisms
1. Vutskits, L. & Xie, Z. Lasting impact of general anaesthesia on the brain: mechanisms and relevance. Nat. Rev. Neurosci. 17, 705–717 (2016).
Review of Pediatric Pain Assessment
1. Cohen, L. L., Lemanek, K., Blount, R. L., Dahlquist, L. M., Lim, C. S., Palermo, T. M., McKenna, K. D., and Weiss, K. E. (2008) Evidence-based assessment of pediatric pain. J Pediatr Psychol. 33, 939–55– discussion 956–7
Mitochondrial Analysis
Shape Quantification
Membrane Potential Analysis
Measuring Cellular ROS
Biological Data Interpretation/Networks
Software
1. Warde-Farley, D. et al. The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function. Nucleic Acids Res 38, W214–20 (2010).
R Resources
Notes from Ben Bolker (McMaster):
https://bbolker.github.io/mixedmodels-misc/glmmFAQ.html
GLMM Course material (more info also on Ben Bolker's github):
https://bbolker.github.io/morelia_2018/
R packages on CRAN (check out vignettes section for tutorials):
Model fitting:
https://cran.r-project.org/web/packages/lme4/index.html
https://cran.r-project.org/web/packages/glmmTMB/index.html
Estimates and inference:
https://cran.r-project.org/web/packages/emmeans/index.html
Residuals for more complex models (glmer and heterogeneous variance fits):
https://cran.r-project.org/web/packages/DHARMa/vignettes/DHARMa.html
Lab Methods
Cancer/Focal Adhesion
1. Chakraborty, S., Lakshmanan, M., Swa, H. L. F., Chen, J., Zhang, X., Ong, Y. S., Loo, L. S., Akıncılar, S. C., Gunaratne, J., Tergaonkar, V., Hui, K. M., and Hong, W. (2015) An oncogenic role of Agrin in regulating focal adhesion integrity in hepatocellular carcinoma. Nat Commun. 6, 6184
Protein Proximity Labeling
1. Bar, D. Z., Atkatsh, K., Tavarez, U., Erdos, M. R., Gruenbaum, Y., and Collins, F. S. (2018) Biotinylation by antibody recognition—a method for proximity labeling. Nat Methods. 15, 127–133
Reproducible TAC
Data That Can (But Won't) Be Used by Hospital Administrators
The Futility of Inane OR Dress Codes
1. Farach, S. M., Kelly, K. N., Farkas, R. L., Ruan, D. T., Matroniano, A., Linehan, D. C., and Moalem, J. (2018) Have Recent Modifications of Operating Room Attire Policies Decreased Surgical Site Infections? An American College of Surgeons NSQIP Review of 6,517 Patients. J Am Coll Surg. 226, 804–813
Why Checklists Shouldn't Be Followed Blindly and Aren't Always the Answer
1. Tamburini, F. B., Andermann, T. M., Tkachenko, E., Senchyna, F., Banaei, N., and Bhatt, A. S. (2018) Precision identification of diverse bloodstream pathogens in the gut microbiome. Nat Med. 4, 278
Our More Recent Papers
Molecular Effects of Anesthesia
8. Plakhotnik, J., Zhang, L., Estrada, M., Coles, J. G., Lonnqvist, P.-A., and Maynes, J. T. (2022) Local Anesthetic Cardiac Toxicity Is Mediated by Cardiomyocyte Calcium Dynamics. Anesthesiology. 137, 687–703
7. Hogarth, K., Vanama, R. B., Stratmann, G., and Maynes, J. T. (2021) Singular and short-term anesthesia exposure in the developing brain induces persistent neuronal changes consistent with chronic neurodegenerative disease. Sci Rep. 11, 5673
6. Petre, M.-A., Levin, D. N., Englesakis, M., Maynes, J. T., Pechlivanoglou, P., and Aoyama, K. (2021) Dexmedetomidine vs. total intravenous anaesthesia in paediatric emergence delirium: A network meta-analysis. European Journal of Anaesthesiology | EJA
5. Echaniz, G., Chan, V., Maynes, J. T., Jozaghi, Y. & Agur, A. Ultrasound-guided maxillary nerve block: an anatomical study using the suprazygomatic approach. Canadian Journal of Anesthesia/Journal canadien d’anesthésie (2019). doi:10.1007/s12630-019-01481-x
4. O'Leary, J. D., Janus, M., Duku, E., Wijeysundera, D. N., To, T., Li, P., Maynes, J. T., Faraoni, D., and Crawford, M. W. (2018) Influence of Surgical Procedures and General Anesthesia on Child Development Before Primary School Entry Among Matched Sibling Pairs. JAMA Pediatr. 173, 29
3. Coghlan, M., Richards, E., Shaik, S., Rossi, P., Vanama, R. B., Ahmadi, S., Petroz, C., Crawford, M., and Maynes, J. T. (2018) Inhalational Anesthetics Induce Neuronal Protein Aggregation and Affect ER Trafficking. Sci Rep. 8, 5275
2. Selvadurai, S., Maynes, J. T., McDonnell, C., Cushing, S. L., Propst, E. J., Lorenzo, A., Lim, A., Meltzer, L. J., Lu, Z., Horner, R. L., and Narang, I. (2018) Evaluating the Effects of General Anesthesia on Sleep in Children Undergoing Elective Surgery: An Observational Case-Control Study. Sleep. 10.1093/sleep/zsy094
1. O'Leary, J. D., Janus, M., Duku, E., Wijeysundera, D. N., To, T., Li, P., Maynes, J. T., and Crawford, M. W. (2016) A Population-based Study Evaluating the Association between Surgery in Early Life and Child Development at Primary School Entry. Anesthesiology. 125, 272–279
General and Cardiac Therapeutic Discovery
10. Drost, C. C., Rovas, A., Kusche-Vihrog, K., Slyke, P. V., Kim, H., Hoang, V. C., Maynes, J. T., Wennmann, D. O., Pavenstädt, H., Linke, W., Lukasz, A., Hesse, B., and Kümpers, P. (2019) Tie2 Activation Promotes Protection and Reconstitution of the Endothelial Glycocalyx in Human Sepsis. Thromb Haemost. 10.1055/s-0039-1695768.
9. Chatterjee, D., Fatah, M., Akdis, D., Spears, D. A., Koopmann, T. T., Mittal, K., Rafiq, M. A., Cattanach, B. M., Zhao, Q., Healey, J. S., Ackerman, M. J., Bos, J. M., Sun, Y., Maynes, J. T., Brunckhorst, C., Medeiros-Domingo, A., Duru, F., Saguner, A. M., and Hamilton, R. M. (2018) An autoantibody identifies arrhythmogenic right ventricular cardiomyopathy and participates in its pathogenesis. European Heart Journal. 39, 3932–3944
8. Traister, A., Patel, R., Huang, A., Patel, S., Plakhotnik, J., Lee, J. E., Medina, M. G., Welsh, C., Ruparel, P., Zhang, L., Friedberg, M., Maynes, J., and Coles, J. (2018) Cardiac regenerative capacity is age- and disease-dependent in childhood heart disease. PLoS ONE. 13, e0200342
7. Wang, L., Dou, W., Malhi, M., Zhu, M., Liu, H., Plakhotnik, J., Xu, Z., Zhao, Q., Chen, J., Chen, S., Hamilton, R., Simmons, C. A., Maynes, J. T., and Sun, Y. (2018) A Micro Device Platform for Continuous Measurement of Contractility, Beating Rate, and Beating Rhythm of hiPSC-Cardiomyocytes inside a Controlled Incubator Environment. ACS Appl Mater Interfaces. 10.1021/acsami.8b05407
6. Gutbier, B., Jiang, X., Dietert, K., Ehrler, C., Lienau, J., Slyke, P. V., Kim, H., Hoang, V. C., Maynes, J. T., Dumont, D. J., Gruber, A. D., Weissmann, N., Mitchell, T. J., Suttorp, N., and Witzenrath, M. (2017) Vasculotide reduces pulmonary hyperpermeability in experimental pneumococcal pneumonia. Critical Care. 21, 274
5. Traister, A., Lu, M., Coles, J. G., and Maynes, J. T. (2016) Dataset of Integrin-linked Kinase Protein:Protein Interactions in Cardiomyocytes Identified by Mass Spectrometry. Data in Brief. 10.1016/j.dib.2016.03.056
4. Sittampalam, S., Eglen, R., Ferguson, S., Maynes, J. T., Olden, K., Schrader, L., Shelper, T., and Ferrer, M. (2015) Three-Dimensional Cell Culture Assays: Are They More Predictive of In Vivo Efficacy than 2D Monolayer Cell-Based Assays? Assay Drug Dev Technol. 13, 254–261
3. Zhu, J., Ide, H., Fu, Y. Y., Teichert, A.-M., Kato, H., Weisel, R. D., Maynes, J. T., Coles, J. G., and Caldarone, C. A. (2014) Losartan ameliorates “upstream” pulmonary vein vasculopathy in a piglet model of pulmonary vein stenosis. J. Thorac. Cardiovasc. Surg. 10.1016/j.jtcvs.2014.07.050
2. Traister, A., Li, M., Aafaqi, S., Lu, M., Arab, S., Radisic, M., Gross, G., Guido, F., Sherret, J., Verma, S., Slorach, C., Mertens, L., Hui, W., Roy, A., Delgado-Olguín, P., Hannigan, G., Maynes, J. T., and Coles, J. G. (2014) Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function. Nat Commun. 5, 4533
1. Kato, H., Fu, Y. Y., Zhu, J., Wang, L., Aafaqi, S., Rahkonen, O., Slorach, C., Traister, A., Leung, C. H., Chiasson, D., Mertens, L., Benson, L., Weisel, R. D., Hinz, B., Maynes, J. T., Coles, J. G., and Caldarone, C. A. (2013) Pulmonary vein stenosis and the pathophysiology of “upstream” pulmonary veins. J. Thorac. Cardiovasc. Surg. 10.1016/j.jtcvs.2013.08.046
Energetics/Mitochondrial Function
4. Toyama, Y., Harkness, R. W., Lee, T. Y. T., Maynes, J. T., and Kay, L. E. (2021) Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR. Proc Natl Acad Sci USA. 118, e2025022118
3. Tong, J., Krieger, J. R., Taylor, P., Bagshaw, R., Kang, J., Jeedigunta, S., Wybenga-Groot, L. E., Zhang, W., Badr, H., Mirhadi, S., Pham, N.-A., Coyaud, É., Yu, M., Li, M., Cabanero, M., Raught, B., Maynes, J. T., Hawkins, C., Tsao, M. S., and Moran, M. F. (2020) Cancer proteome and metabolite changes linked to SHMT2. PLOS ONE. 15, e0237981
2. Abrahamson, J. R. et al. Renal tissue Po2 sensing during acute hemodilution is dependent on the diluent. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 318, R799–R812 (2020).
1. Hogarth, K. A., Costford, S. R., Yoon, G., Sondheimer, N., and Maynes, J. T. (2017) DNM1L Variant Alters Baseline Mitochondrial Function and Response to Stress in a Patient with Severe Neurological Dysfunction. Biochem Genet. 7, 1–22
Pediatric Medicine/Molecular Basis of Disease
23. Diskin, C., Orkin, J., Dharmaraj, B., Agarwal, T., Parmar, A., McNaughton, K., Cohen, E., Sunderji, A., Faraoni, D., Fecteau, A., Fischer, J., Maynes, J., Mahant, S., and Friedman, J. (2023) Secondary impacts of the COVID-19 pandemic at a tertiary children’s hospital in Canada: a mixed-methods study. BMJ Open. 13, e059849, doi: 10.1136/bmjopen-2021-059849.
22. Saleh, E., Pechhacker, M. G., Vig, A., Mehta, M., Maynes, J., Tumber, A., Tavares, E., Vincent, A., Mireskandari, K., and Heon, E. (2022) Unilateral cataract and congenital stationary night blindness in a child with novel variants in TRPM1. Journal of American Association for Pediatric Ophthalmology and Strabismus {JAAPOS}. doi: 10.1016/j.jaapos.2022.03.013
21. Grudzinska Pechhacker, M. K., Jacobson, S. G., Drack, A. V., Scipio, M. D., Strubbe, I., Pfeifer, W., Duncan, J. L., Dollfus, H., Goetz, N., Muller, J., Vincent, A. L., Aleman, T. S., Tumber, A., Van Cauwenbergh, C., De Baere, E., Bedoukian, E., Leroy, B. P., Maynes, J. T., Munier, F. L., Tavares, E., Saleh, E., Vincent, A., and Heon, E. (2021) Comparative Natural History of Visual Function From Patients With Biallelic Variants in BBS1 and BBS10. Investigative Ophthalmology & Visual Science. 62, 26–26
20. Shao, Z., Masuho, I., Tumber, A., Maynes, J. T., Tavares, E., Ali, A., Hewson, S., Schulze, A., Kannu, P., Martemyanov, K. A., and Vincent, A. (2021) Extended Phenotyping and Functional Validation Facilitate Diagnosis of a Complex Patient Harboring Genetic Variants in MCCC1 and GNB5 Causing Overlapping Phenotypes. Genes. 10.3390/genes12091352
19. Shioji, N., Aoyama, K., Englesakis, M., Annich, G., and Maynes, J. T. (2021) Multisystem inflammatory syndrome in children during the coronavirus disease pandemic of 2019: a review of clinical features and acute phase management. J Anesth. 10.1007/s00540-021-02952-6
18. Gai, N., Aoyama, K., Faraoni, D., Goldenberg, N. M., Levin, D. N., Maynes, J. T., McVey, M. J., Munshey, F., Siddiqui, A., Switzer, T., and Steinberg, B. E. (2021) General medical publications during COVID-19 show increased dissemination despite lower validation. PLOS ONE. 16, e0246427
17. Tabbarah, S., Tavares, E., Charish, J., Vincent, A., Paterson, A., Di Scipio, M., Yin, Y., Mendoza-Londono, R., Maynes, J., Heon, E., and Monnier, P. P. (2020) COG5 variants lead to complex early onset retinal degeneration, upregulation of PERK and DNA damage. Sci Rep. 10, 21269
16. Grudzinska Pechhacker, M. K., Yoon, G., Hazrati, L.-N., Maynes, J., MacDonald, H., Tavares, E., Vincent, A., and Heon, E. (2020) FLVCR1-related disease as a rare cause of retinitis pigmentosa and hereditary sensory autonomic neuropathy. European Journal of Medical Genetics. 10.1016/j.ejmg.2020.104037
15. Gai, N., Maynes, J. T., and Aoyama, K. (2020) Unique challenges in pediatric anesthesia created by COVID-19. J Anesth. 10.1007/s00540-020-02837-0.
14. Daeschler, S. C., Manson, N., Joachim, K., Chin, A. W. H., Chan, K., Chen, P. Z., Jones, A., Tajdaran, K., Mirmoeini, K., Zhang, J. J., Maynes, J. T., Zhang, L., Science, M., Darbandi, A., Stephens, D., Gu, F., Poon, L. L. M., and Borschel, G. H. (2020) Effect of moist heat reprocessing of N95 respirators on SARS-CoV-2 inactivation and respirator function. CMAJ. 10.1503/cmaj.201203
13. Vig, A., et. al., and Genomics England Research Consortium (2020) DYNC2H1 hypomorphic or retina-predominant variants cause nonsyndromic retinal degeneration. Genetics in Medicine. 10.1038/s41436-020-0915-1
12. Aoyama, K., Heath, A., Yang, A., Maynes, J. T., Petroz, G., Robertson, J., Mc Donnell, C., Velummailum, R., Bond, E., and Pechlivanoglou, P. (2020) Estimating the risk of SARS-CoV-2 transmission to pediatric anesthesiologists: a microsimulation model. Can J Anesth/J Can Anesth. 10.1007/s12630-020-01771-9
11. Steinberg, B. E., Aoyama, K., McVey, M., Levin, D., Siddiqui, A., Munshey, F., Goldenberg, N. M., Faraoni, D., and Maynes, J. T. (2020) Efficacy and safety of decontamination for N95 respirator reuse: a systematic literature search and narrative synthesis. Can J Anesth/J Can Anesth. 10.1007/s12630-020-01770-w
10. Kletke, S. N., Vincent, A., Maynes, J. T., Elbaz, U., Mireskandari, K., Lam, W.-C., and Ali, A. (2020) A de novo mutation in PITX2 underlies a unique form of Axenfeld-Rieger syndrome with corneal neovascularization and extensive proliferative vitreoretinopathy. Ophthalmic Genetics. 10.1080/13816810.2020.1768556.
9. Shao, Z., Tumber, A., Maynes J.T., Tavares, E., Kannu, P., Heon, E. and Vincent, A. (2019) Unique retinal signaling defect in GNB5-related disease. Doc Ophthalmol 10.1007/s10633-019-09735-1.
8. Lokoff, A., and Maynes, J. T. (2019) The incidence, significance, and management of accidental intra-arterial injection: a narrative review. Can J Anaesth. 10.1007/s12630-019-01327-6
7. Norris, M. J., Malhi, M., Duan, W., Ouyang, H., Granados, A., Cen, Y., Tseng, Y.-C., Gubbay, J., Maynes, J., and Moraes, T. J. (2018) Targeting Intracellular Ion Homeostasis for the Control of Respiratory Syncytial Virus. Am. J. Respir. Cell Mol. Biol. 10.1165/rcmb.2017-0345OC
6. Moran, J., Sanderson, K. G., Maynes, J., Vig, A., Batmanabane, V., Kannu, P., Tavares, E., Vincent, A., and Heon, E. (2018) IFT80 mutations cause a novel complex ciliopathy phenotype with retinal degeneration. Clin. Genet. 10.1111/cge.13408
5. Wong, B. M., Keilman, J., Zuccaro, J., Kelly, C., Maynes, J. T., and Fish, J. S. (2016) Anesthetic Practices for Laser Rehabilitation of Pediatric Hypertrophic Burn Scars. Journal of Burn Care & Research. Publish Ahead of Print, 1
4. Vincent, A., Ng, J., Gerth-Kahlert, C., Tavares, E., Maynes, J. T., Wright, T., Tiwari, A., Tumber, A., Li, S., Hanson, J. V. M., Bahr, A., MacDonald, H., Bähr, L., Westall, C., Berger, W., Cremers, F. P. M., Hollander, den, A. I., and Heon, E. (2016) Biallelic Mutations in CRB1Underlie Autosomal Recessive Familial Foveal Retinoschisis. Invest. Ophthalmol. Vis. Sci. 57, 2637–2646
3. Vincent, A., Audo, I., Tavares, E., Maynes, J. T., Tumber, A., Wright, T., Li, S., Michiels, C., Condroyer, C., MacDonald, H., Verdet, R., Sahel, J.-A., Hamel, C. P., Zeitz, C., and Heon, E. (2016) Biallelic Mutations in GNB3 Cause a Unique Form of Autosomal-Recessive Congenital Stationary Night Blindness. The American Journal of Human Genetics. 10.1016/j.ajhg.2016.03.021
2. Vincent, A., Forster, N., Maynes, J. T., Paton, T. A., Billingsley, G., Roslin, N. M., Ali, A., Sutherland, J., Wright, T., Westall, C. A., Paterson, A. D., Marshall, C. R., Consortium, F. C., and Heon, E. (2014) OTX2 mutations cause autosomal dominant pattern dystrophy of the retinal pigment epithelium. J Med Genet. 10.1136/jmedgenet-2014-102620
1. Skene-Arnold, T. D., Luu, H. A., Uhrig, R. G., De Wever, V., Nimick, M., Maynes, J., Fong, A., James, M. N. G., Trinkle-Mulcahy, L., Moorhead, G. B., and Holmes, C. F. B. (2013) Molecular mechanisms underlying the interaction of protein phosphatase-1c with ASPP proteins. Biochemical Journal. 449, 649–659